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On the development of a test for the selection of shift workers for work in the North based on the determination of the metabolomic profile of blood plasma, by the example of workers in water transport

O.N. Kolosova1,*, E.Z. Zasimova2, I.V. Sleptsov1, A.S. Golderova2B.M. Kershengolts1
DOI 10.31242/2618-9712-2021-26-4-91-102

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1Institute for Biological Problems of Cryolithozone SB RAS, Yakutsk, Russia
2M.K. Ammosov North-Eastern Federal University, Yakutsk, Russia
*[email protected]

Received 06.10.2021
Accepted 11.11.2021

Citation
Kolosova O.N., Zasimova E.Z., Sleptsov I.V., Golderova A.S., Kershengolts B.M. On the development of a test for the selection of shift workers for work in the North based on the determination of the metabolomic profile of blood plasma, by the example of workers in water transport // Arctic and Subarctic Natural Resources. 2021, Vol. 26, No. 4. P. 91–102. (In Russ.) https://doi.org/10.31242/2618-9712-2021-26-4-91-102

Abstract. A prospective cohort study of the influence of a long navigation period on the metabolomic blood plasma profile of shift workers (river workers) in the Far North was carried out. On the basis of voluntary written informed consent, 53 water transport workers of the Republic of Sakha (Yakutia), who arrived from other regions of Russia and the CIS, took part in the study. The survey was carried out before (spring) and after (autumn) the voyage. The identification and determination of the concentrations of metabolites in blood plasma samples was carried out by means of gas chromatography with mass spectrometry (GC-MS). Analysis of blood plasma metabolomic profile data was performed using the MetaboAnalyst platform (V5.0). The results of studies of the metabolomic profile of blood plasma in river workers revealed significant differences in both the qualitative and quantitative composition of metabolites before the beginning of navigation (spring) and after its end (autumn). In autumn, after long-term navigation, the proportion of river workers with a breakdown in adaptation increases by a factor of 1.81, which indicates a significant decrease in adaptation reserves during navigation. The level of 5-hydroxyproline in the blood of this group of river workers increases. The increased content of ribonic acid in blood serum in spring is the evidence of the high adaptive potential of a person, the ability of the body to work out a long shift in the northern river fleet without depleting the adaptive reserve, that is, without prejudice to health. The results obtained make it possible to propose a metabolomic test, first of all, the content of ribonic acid in blood plasma, as a test for the selection of shift workers for work in the Arctic and Subarctic.

Keywords: adaptation, extreme conditions, shift work, water transport, blood plasma metabolomic profile, principal component analysis, the Arctic, GC-MS.

Acknowledgements. The research was carried out within the framework of the following projects: «Research of chronophysiological, molecular adaptation mechanisms and psychoemotional stress of a person under the conditions of extreme climatic, technogenic factors in the Arctic and Subarctic and a pandemic, post-pandemic, development of ways to increase the adaptive potential» (No. FWRS-2021-0043 registration number 121021600269-0) Federal Research Center «YSC SB RAS»; «Physiological and biochemical adaptation mechanisms of plants, animals, humans to the conditions of the Arctic / Subarctic and the devel-
opment of biological products based on natural northern raw materials that increase the efficiency of the adaptation process and the level of human health under extreme environmental conditions» (No. 02972021-0025 registration number AAAA-A21 -121012190035-9) Institute of Biological Problems of Cryolithozone «FRC YSC SB RAS»; project No. 6 KNI-2 «Development and implementation in practical health care of biogenic methods of increasing the level of health and adaptive potential of the human body, its mental and physical performance under extreme conditions of the natural and social environment by creating biological products from the tissues of northern ecoforms of plants and aboriginal animal species»; a technological project of the World Class Scientific and Educational Center: North: Territory of Sustainable Development No. 8 «Biotechnology of deep processing of the unique northern, environmentally friendly, reproducible biological raw materials.»


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